Detection of polyamines by a new enzymatic differential assay. (4) . Fundamental study on a new enzymatic differential assay of blood
Koide, T.; Sakai, S.; Takeda, A.; Doi, T.; Tei, K.; Hara, A.; Sawada, H.
Hinyokika Kiyo. Acta Urologica Japonica 32(7): 913-917
1986
ISSN/ISBN: 0018-1994 PMID: 3776770 Document Number: 281963
The enzymatic method for isolation and determination of urinary polyamines was modified to measure the polyamines in the blood. High recovery rates of polyamine in blood by enzymatic hydrolyzation were obtained, namely, 101.9 +/- 4.4% for diamine, 96.0 +/- 5.4% for spermidine and 104.1 +/- 3.3% for spermine. Furthermore excellent linearity was demonstrated. Within-run precision of polyamine in blood was excellent, namely, in C.V., 1.15% for Reaction 1, 2.11% for Reaction 2 and 2.79% for Reaction 3. This method was compared with high pressure liquid chromatography (HPLC), and a close correlation was demonstrated for all the fractions: diamine r = 0.8824, y = 1.367x+0.0417 (n = 15); spermidine r = 0.9878, y = 0.806x+5.218 (n = 15); spermine r = 0.9764, y = 1.068x-0.9195 (n = 15).