Quantitative enzymatic assay of short-chain acylcarnitines after separation by paper chromatography

Golan, R.; Lewin, L.M.

Analytical Biochemistry 105(2): 264-267

1980


ISSN/ISBN: 0003-2697
PMID: 7192940
Document Number: 162234
Esters of carnitine with fatty acids containing 2 to 5 atoms were separated by descending chromatography on Whatman No. 1 paper, using the solvent system, n-butanol:acetic acid:water (8:1:1, vol/vol). The separated derivatives were hydrolyzed with ammonia; the resulting L-carnitine was eluted from each chromatographic zone and assayed enzymatically. This procedure permits separation and assay of nanomole amounts of acylcarnitines from biological materials.

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