Methylation patterns of tRNA at different concentrations of dimethylsulphate

Kanduc, D.

Bollettino della Societa Italiana di Biologia Sperimentale 55(22): 2372-2375

1979


ISSN/ISBN: 0037-8771
PMID: 398704
Document Number: 142467
The methylation patterns produced in E. coli B tRNA by various concentrations of dimethylsulphate were found to differ with a predominant formation of 7-methylguanine and 1-methyladenine at low concentrations and of a methylated compound not yet identified at high concentrations of methylating agent. The analysis by Scatchard plot of dimethylsulphate interaction with the nucleic acid suggested the presence of high and low affinity sites.

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