Recovery of CFU-C after freezing of normal and leukemic human bone marrow
Robinson, W.A.; Drebing, C.; Mahmood, T.; Entringer, M.; Redecker, S.
Experimental Hematology 7 Suppl. 5: 27-37
1979
ISSN/ISBN: 0301-472X PMID: 400689 Document Number: 141142
Studies have been carried out to determine the viability of leukemic and normal human bone marrow, cryopreserved in liquid nitrogen at -196 degrees C, using changes in total cell numbers and granulocyte colony forming ability in vitro. These studies have shown that there is considerable variability in the recovery of CFU-C from individual specimens. When the overall recovery, in all patients, is taken into account, there is a gradual decline in CFU-C numbers to about 60% after 24 months of freezing. CFU-C recovery is closely correlated with recovery of total cell numbers.