In vitro and in vivo recovery of marrow progenitor cells after cryopreservation and autotransplantation of the bone marrow
Dragani, A.; Iacone, A.; Accorsi, P.; Angelini, A.; Di Girolamo, G.; Geraci, L.; Berardi, A.; D'Antonio, D.; Di Bartolomeo, P.; Cacciafiori, G.
Bollettino della Societa Italiana di Biologia Sperimentale 63(3): 217-224
1987
ISSN/ISBN: 0037-8771 PMID: 3307836 Document Number: 293513
In the last years high doses of chemotherapy with or without total body irradiation followed by autologous marrow rescue have had a fundamental role in the treatment of malignancies. From February 1982 to July 1984, 22 bone marrow have been harvested from patients affected by acute leukemia (9 cases), chronic myelogenous leukemia (4), lymphoma (4), neuroblastoma (3), small cells bronchogenic carcinoma (1) and normal hematological allogenic donor (1). The collected heparinized marrow was filtered and concentrated with a blood processor (IBM 2991). After dilution with 20% DMSO and 80 cryoprecipitate and poor plasma platelets, the nucleated marrow derived cells were frozen in an automated controlled freezer and stored in liquid nitrogen. For in vitro test, 2 .times. 10e5 thawed and washed cells were cultured in the double-layer semi-solid agar plate with incubation for 7-10 days in a fully humified atmosphere of 5% CO2. Recovery of CFU-GM ranged between 50 and 100% with a mean of 92 .+-. 33,5. In three cases cryopreserved marrow was reinfused and was able to restore hemopoiesis after heavy conditioning regimen consisting in 120 mg/Kg of cyclophosphamide and 10 Gy of fractionated (2 Gyx5) total body irradiation.