Effect of insulin, dexamethasone, and glucagon on the amino acid transport ability of four rat hepatoma cell lines and rat hepatocytes in culture
Kelley, D.S.; Becker, J.E.; Potter, V.R.
Cancer Research 38(12): 4591-4600
1978
ISSN/ISBN: 0008-5472 PMID: 214227 Document Number: 138918
The basal level and hormone-affected amino acid transport of rat hepatocytes and rat hepatoma cell lines H4-II-E-C3 (H35), HTC, McA-RH 8994 (8994), and McA-RH 7777 (7777) in culture were examined by investigating the amount of .alpha.-AMP. Without any hormone treatment cell line 7777 exhibited the maximum rate of AIB uptake followed by cell lines HTC, 8994, H35 and hepatocytes. Insulin increased the uptake of AIB by hepatocytes as well as by all 4 of the hepatoma cell lines. DEX increased AIB transport in cell lines H-35 and 8994 and inhibited it in hepatocytes and hepatoma cell lines HTC and 7777. The effect of insulin and DEX was additive in cell lines H35 and 8994 and was antagonistic in hepatocytes and cell lines HTC and 7777. In contrast to their stimulatory effect on AIB transport in rat liver parenchymal cells, glucagon or dibutyryl cAMP had no effect on the transport system of any of these 4 cell lines. The potentiation of AIB transport by DEX, in response to glucagon or dibutyryl cAMP as seen in rat liver parenchymal cells, could not be observed in any of these 4 cell lines. Both actinomycin D and cycloheximide blocked any of the effects of insulin, DEX, or glucagon in all of these cell culture types. In spite of several similarities there apparently is a marked heterogeneity in hormone responses among the various hepatoma cell lines although each differs in some respect from the hepatocytes.