The effect of EBNA-1 ribozyme on the ability of tumor genesis of lymphoblastoid cell lines in SCID mice
Liu, A.; Jiang, Y.; Zhao, K.
Zhonghua Yi Xue Za Zhi 79(7): 538-541
1999
ISSN/ISBN: 0376-2491 PMID: 11715427 Document Number: 508205
To explore the effect of EBNA-1 specific ribozyme on the tumor genesis ability of lymphoblastoid cell lines (LCLs) in severe combined immunodeficiency (SCID) mice. Using molecular cloning technique, recombinant adenovirus vectors expressing EBNA-1 ribozyme (RZ1) or its inactive mutant (RZ1mut) were constructed as Ad.RZ1 or Ad.RZ1mut. Recombinant adenoviruses were generated by homologous recombination and isolated by plaque formation. LCLs were established and infected with recombinant adenovirus and then injected in SCID mice (1 x 10(7) cells/animal). The SCID mice were sacrificed six weeks after injection, then the tumors were excised from these mice and their size and weight were measured. The expression of EBNA-1 ribozyme and EBNA-1 mRNA in the tumor was analyzed by RT-PCR, and the expression of EBNA-1 protein in the tumor was detected by Western blot. The tumors (0.27 +/- 0.18) g formed from the Ad.RZ1-treated LCLs were much smaller than those of the untreated LCLs (1.04 +/- 0.27) g or LCLs treated with control vector of adenovirus (1.12 +/- 0.32) g (P < 0.01), and also smaller than those of Ad.RZ1mut-treated LCLs (0.76 +/- 0.28) g (P < 0.05). The expression of EBNA-1 mRNA and protein in the tumors of Ad.RZ1-treated LCLs decreased significantly than that of other groups. Adenovirus delivery of EBNA-1 ribozyme was able to suppress LCL tumors significantly and depress the tumor genesis ability of B lymphocyte induced by EBV.