Stereospecific antibodies to methadone. II. Synthesis of d- and 1-methadone antigens
Bartos, F.; Bartos, D.; Olsen, G.D.; Anderson, B.; Daves, G.D.
Research Communications in Chemical Pathology and Pharmacology 20(1): 157-164
1978
ISSN/ISBN: 0034-5164 PMID: 566457 Document Number: 137702
Diastereomeric methadols were prepared by reduction of the methadone enantiomers. Resulting methadols, after purification and characterization by mass spectrometry, were succinylated. The hemisuccinyl derivatives were conjugated with thyroglobulin via carbodiimide condensation. The conjugates of d- and of 1-methadol hemisuccinyl-thyroglobulin were employed as the antigens for immunization of white New Zealand rabbits in order to obtain specific anti d- and anti 1-methadone antisera.