Changes in erythrocyte membrane permeability after freezing with polyethylene oxide and glycerol
Osipenko, A.P.; Belous, A.M.; Lishko, V.K.
Ukrainskii Biokhimicheskii Zhurnal 50(3): 364-377
1978
ISSN/ISBN: 0201-8470 PMID: 664051 Document Number: 136464
Membrane ion permeability to sodium cations and phosphate anions, acid resistance and phospholipid composition were studied in erythrocytes frozen and thawed with 15% glycerol and 10% solution of polyethylene oxide 1500. An increase in membrane permeability to 22Na and HPO4(2-) against a background of reduction in phospholipids and total acid resistance was observed after rapid freezing-thawing and removing of cryoprotectants.