Measurement of acid phosphatase activity in various tissues and cultured cells
Kaighn, M.E.
National Cancer Institute Monograph 49: 59-60
1978
ISSN/ISBN: 0083-1921 PMID: 86169 Document Number: 132151
Acid phosphatase activity has been measured in various tissues and in extracts of cultured cells. Cells were solubilized with NP40 at neutral pH, and thymolphthalein monophosphate was used as substrate. Reproducible assays were possible with a few as 10(6) cells.
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