A reference method for measurement of alkaline phosphatase activity in human serum
Tietz, N.W.; Burtis, C.A.; Duncan, P.; Ervin, K.; Petitclerc, C.J.; Rinker, A.D.; Shuey, D.; Zygowicz, E.R.
Clinical Chemistry 29(5): 751-761
1983
ISSN/ISBN: 0009-9147 PMID: 6404566 Document Number: 217856
We present an official AACC reference method for the measurement of alkaline phosphatase, the culmination of optimization experiments conducted by a group of independent laboratories. The details of this method and evaluation of factors affecting the measurement are described. A metal ion buffer has been incorporated that maintains optimal and constant concentrations of zinc(II) and magnesium(II) ions. Final reaction conditions are: pH (30 degrees C), 10.40 +/- 0.05; 2-amino-2-methyl-1-propanol buffer, 0.35 mol/L; 4-nitrophenyl phosphate, 16.0 mmol/L; magnesium acetate, 2.0 mmol/L; zinc sulfate, 1.0 mmol/L; and N-(2-hydroxyethyl)ethylenediaminetriacetic acid, 2.0 mmol/L.