Effects of high fat and high carbohydrate diets on the body composition and oxygen consumption of ob/ob mice
Fanelli, M.T.; Kaplan, M.L.
Journal of Nutrition 108(9): 1491-1500
1978
ISSN/ISBN: 0022-3166 PMID: 682052 Document Number: 132011
The effects of high fat and high carbohydrate diets on the development of body composition and O2 consumption of genetically obese (C57BL/6J ob/ob) mice and non-obese (non-ob/ob) littermates were reported. The genotype of individual mice was identified on day 18 on the basis of values of O2 consumption. Non-ob/ob and ob/ob mice were fed ad lib with either a high carbohydrate or high fat diet, which were identical in energy and protein content, from weaning on day 21 until 42 days of age. The non-ob/ob mice did not exhibit differences in food consumption when fed either diet. By day 42 the total protein and ash accretion of the non-ob/ob mice were similar when fed both diets while the total lipid accretion was decreased with the high fat diet. The ob/ob mice exhibited an increased food consumption when fed the high fat diet and deposited more total protein, lipid and ash by day 42 than the high carbohydrate fed ob/ob mice. O2 consumption of the mice was determined at days 21, 24, 28, 35 and 42, and day 18. The O2 consumption of all the mice tended to decrease from day 21 to day 42. The decrease in O2 consumption was significant only for the carbohydrate-fed males of both genotypes from day 21-24. The high carbohydrate diet also tended to decrease the O2 consumption among the females of both genotypes. The high fat diet tended to raise the postweaning values of O2 consumption of non-ob/ob and ob/ob male mice towards the preweaning values. This suggests that the usually observed postweaning reduction in O2 consumption may be attributed to the shift from a high fat to a high carbohydrate diet, and other developmental changes that occur at weaning. The high fat-fed mice tended to consume more O2 than the high carbohydrate-fed mice. The increased O2 consumption may be related to increased oxidation of total energy. The changes in the body composition of non-ob/ob mice reflect the increased energy expenditure of the high fat-fed non-ob/ob mice.