Template properties of the decadeoxynucleotide d (pCCACGAAACC) in the RNA polymerase system of Escherichia coli
Badashkeeva, A.G.; Denisova, L.Ia.; Zagrebel'nyĭ, S.N.; Knorre, D.G.; Pustoshilova, N.M.
Molekuliarnaia Biologiia 12(2): 327-333
1978
ISSN/ISBN: 0026-8984 PMID: 349360 Document Number: 129847
The decadeoxynucleotide d(pCCACGAAACC) transcription by E. coli RNA-polymerase was studied. The transcript was shown to be several times longer than the template. The oligonucleotide GpGpGpGpUp complementary to the "contact" of two neighbouring template molecules was found in the pancreatic RNase digest of the RNA-product. This fact is consistent with our hypothesis reported recently. Pentanucleotide d(pGGTTT) may funtion as a primer in the decadeoxynucleotide transcription being incorporated into RNA.