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Role of RNA polymerase in ensuring fidelity in copying the template during transcription in E. coli

Kamzolova, S.G.; Ozolin', O.N.

Biokhimiia 46(4): 717-722

1981

ISSN/ISBN: 0320-9725
PMID: 7025923
Document Number: 182255
The heterogeneity of cell size of E. coli WU-36-10-11-12 and its four RNA-polymerase (rif-r) mutants with pleiotropic effect -- rpoB401, rpoB402, rpoB403 and rpoB409 was investigated for the purposeful choice of E. coli mutant with an altered fidelity of transcription. The stability of the phenotype of E. coli strains was shown to depend on the structural state of RNA polymerase. In vitro RNA-polymerase of the morphologically most unstable mutant rpoB402 incorporates non-complementary GMP or CMP on the poly [d(AT).d(AT)] template more frequently than the enzyme from the wild-type strain. The data obtained suggest that the beta-subunit of RNA-polymerase determines the fidelity of transcription and the selection of complementary nucleotides.

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Lymans'kyĭ, O.P. 2007: Visualization of bacteriophage T7 RNA-polymerase complexes with DNA template in the process of transcription elongation Ukrains'kyi Biokhimichnyi Zhurnal 79(1): 94-103
Badashkeeva, A.G.; Denisova, L.Ia.; Zagrebel'nyĭ, S.N.; Knorre, D.G.; Pustoshilova, N.M. 1978: Template properties of the decadeoxynucleotide d(pCCACGAAACC) in the RNA polymerase system of Escherichia coli Molekuliarnaia Biologiia 12(2): 327-333
Shakulov, R.S.; Kliachko, E.V.; Ponomarenko, O.I. 1985: The role of pp Gpp in the coordination of transcription of the ribosomal protein genes rplKAJL and RNA-polymerase rpoBC genes in Escherichia coli cells Molekuliarnaia Biologiia 19(6): 1603-1609
Bruskov, V.I.; Kuklina, O.V. 1988: Display of 8-hydroxy-GTP substrate properties of UTP in the reaction of polynucleotide synthesis catalyzed by RNA-polymerase from Escherichia coli in the presence of poly[d(AT).d(AT)] template Molekuliarnaia Biologiia 22(3): 726-730
Vorob'eva, N.V.; Nebrat, L.T.; Potapov, V.A.; Romashchenko, A.G.; Salganik, R.I. 1982: Reverse transcription of heterologous RNA by RNA-dependent DNA polymerase from Escherichia coli Molekuliarnaia Biologiia 16(5): 977-983
Qi, F.; Liu, C.; Heath, L.S.; Turnbough, C.L. 1996: In vitro assay for reiterative transcription during transcriptional initiation by Escherichia coli RNA polymerase Methods in Enzymology 273: 71-85
Iarulin, V.R.; Gorlenko, Z.M. 1985: Effect of mutation changes in RNA-polymerase and transcription termination factor rho on expression of various operons in E. coli Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 10: 8-19
Schy, W.E.; Hertel, L.W.; Kroin, J.S.; Bloom, L.B.; Goodman, M.F.; Richardson, F.C. 1993: Effect of a template-located 2',2'-difluorodeoxycytidine on the kinetics and fidelity of base insertion by Klenow (3'-->5'exonuclease-) fragment Cancer Research 53(19): 4582-4587
Ottonello, S.; Rivier, D.H.; Doolittle, G.M.; Young, L.S.; Sprague, K.U. 1987: The properties of a new polymerase III transcription factor reveal that transcription complexes can assemble by more than one pathway EMBO Journal 6(7): 1921-1927
Lescure, A.; Lutz, Y.; Eberhard, D.; Jacq, X.; Krol, A.; Grummt, I.; Davidson, I.; Chambon, P.; Tora, L. 1994: The N-terminal domain of the human TATA-binding protein plays a role in transcription from TATA-containing RNA polymerase II and III promoters EMBO Journal 13(5): 1166-1175
Volchkova, V.A.; Gorn, V.V.; Kolocheva, T.I.; Lavrik, O.I.; Levina, A.S. 1989: Klenow fragment of DNA-polymerase i from E. coli. III. the role of internucleotide phosphate groups of the matrix in its binding with the enzyme Bioorganicheskaia Khimiia 15(1): 78-89
Kamzolova, S.G. 1995: Interaction of Escherichia coli RNA polymerase with promoters. The need for a classified approach in studying the code of promoter-polymerase recognition Biokhimiia 60(3): 387-394
Weissmann, G.; Hirschhorn, R. 1970: Effect of trypsin and phytohemagglutinin on template capacity of mammalian nuclei for RNA polymerase Mount Sinai Journal of Medicine new York 37(3): 192-205
Chomczyński, P.; Szafrański, P. 1971: The effect of high salt concentration on fidelity of translation by Escherichia coli ribosomes Acta Biochimica Polonica 18(2): 163-170
Momparler, R.L. 1972: Kinetic and template studies with 1- -D-arabinofuranosylcytosine 5'-triphosphate and mammalian deoxyribonucleic acid polymerase Molecular Pharmacology 8(3): 362-370
Nevinskiĭ, G.A.; Potapova, I.A.; Tarusova, N.B.; Khalabuda, O.V.; Khomov, V.V. 1990: Interaction of dNTP-binding sites of human DNA polymerase alpha and the Klenow fragment of Escherichia coli DNA polymerase i with nucleotides, pyrophosphate and their analogs Molekuliarnaia Biologiia 24(1): 104-116
Abbott, G.F.; Hawkins, S.E. 1980: Informational molecules in amoebae studies of template utilization by the DNA polymerase activities of Amoeba discoides Cytobios 27: 107-108
Podust, V.N.; Korobeĭnicheva, T.O.; Nevinskiĭ, G.A.; Rikhter, V.A.; Abramova, T.I.; Lavrik, O.I. 1990: Template-primer-dependent inactivation of DNA polymerase alpha from human placenta by 2',3'-epoxyadenosine-5'-triphosphate Bioorganicheskaia Khimiia 16(2): 226-235
von der Decken, A.; Andersson, G.M. 1977: Template properties of rat liver chromatin after restricted protein supply; trascription by homologous ribonucleic acid polymerase I and II Journal of Nutrition 107(6): 949-958
Goureau-Counis, M.F.; Fichot, O.; Raulin, J.; De Recondo, A.M. 1974: Phospholipid configuration and template capacity of liver nuclei and purified chromatin in presence or absence of hepatic DNA polymerase Physiological Chemistry and Physics 6(5): 379-392