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The properties of a new polymerase III transcription factor reveal that transcription complexes can assemble by more than one pathway

Ottonello, S.; Rivier, D.H.; Doolittle, G.M.; Young, L.S.; Sprague, K.U.

EMBO Journal 6(7): 1921-1927

1987

ISSN/ISBN: 0261-4189
PMID: 3653074
Document Number: 305252
We have resolved a previously unidentified factor (TFIIID) that is required for in vitro transcription of polymerase III templates. Our ability to resolve factor D from each of the other components of the transcription machinery (polymerase and transcription factors IIIB and IIIC) allowed us to test the capacity of these separated components to form stable complexes with tRNA genes. We find that none of the individual components binds detectably to tRNA genes, but that certain combinations of transcription factors do bind. Our results show that TFIIID is essential for binding and that formation of a full transcription complex can proceed by either of two different pathways.

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Geiduschek, E.P.; Bardeleben, C.; Joazeiro, C.A.; Kassavetis, G.A.; Whitehall, S. 1995: Yeast RNA polymerase III: transcription complexes and RNA synthesis Brazilian Journal of Medical and Biological Research 28(2): 147-159
Lymans'kyĭ, O.P. 2007: Visualization of bacteriophage T7 RNA-polymerase complexes with DNA template in the process of transcription elongation Ukrains'kyi Biokhimichnyi Zhurnal 79(1): 94-103
Liao, F.; Birshtein, B.K.; Busslinger, M.; Rothman, P. 1994: The transcription factor BSAP (NF-HB) is essential for immunoglobulin germ-line epsilon transcription Journal of Immunology 152(6): 2904-2911
Du, H.; Roy, A.L.; Roeder, R.G. 1993: Human transcription factor USF stimulates transcription through the initiator elements of the HIV-1 and the Ad-ML promoters EMBO Journal 12(2): 501-511
Lu, Q.; Yan, J.; Xiong, Y.; Cai, L.; Li, X.-w.; Dong, J.-h. 2009: Hepatitis B virus X protein upregulates the transcription activity of zinc finger transcription factor SNAI1 Zhonghua Yi Xue Za Zhi 89(14): 990-993
Schnapp, G.; Santori, F.; Carles, C.; Riva, M.; Grummt, I. 1994: The HMG box-containing nucleolar transcription factor UBF interacts with a specific subunit of RNA polymerase I EMBO Journal 13(1): 190-199
Iarulin, V.R.; Gorlenko, Z.M. 1985: Effect of mutation changes in RNA-polymerase and transcription termination factor rho on expression of various operons in E. coli Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 10: 8-19
Cohen, D.R.; Vandermark, S.E.; McGovern, J.D.; Bradley, M.P. 1993: Transcriptional regulation in the testis: a role for transcription factor AP-1 complexes at various stages of spermatogenesis Oncogene 8(2): 443-455
Goenechea, L.G.; Rendon, M.C.; Iglesias, C.; Valdivia, M.M. 1992: Immunostaining of nucleolus organizers in mammalian cells by a human autoantibody against the polymerase i transcription factor UBF Cellular and Molecular Biology 38(8): 841-851
Bruno, S.; De Virgilio, C.; Gadaleta, G. 2007: The smaller isoform of the mitochondrial transcription factor a has a role in the mitochondrial transcription Italian Journal of Biochemistry 56(4): 315-318
Alaaeddine, N.; Claudette de Montigny; M'Bark Sadouk 2011: Real-Time Reverse Transcription-Polymerase Chain Reaction Quantification of Tumor Necrosis Factor Alpha Messenger in Human Leukocytes Clinical Laboratory 57(9-10): 799-802
Kozlov, I.A.; Ivanovskaia, M.G.; Kubareva, E.A.; Volkov, E.M.; Shabarova, Z.A. 1996: Synthesis and properties of a covalently-bound DNA-duplex containing a segment that recognizes transcription factor NF-kappa B Molekuliarnaia Biologiia 30(4): 852-863
Kamzolova, S.G.; Kamzaolov, S.S.; Ivanova, N.N. 1998: Conformational changes of a transcription complex of RNA-polymerase with DNA Molekuliarnaia Biologiia 32(5): 788-792
Cieśla, M.łg.; Boguta, M. 2008: Regulation of RNA polymerase IIi transcription by Maf1 protein Acta Biochimica Polonica 55(2): 215-225
Ishihama, A. 1971: Transcription of genetic information. 2. Structure and function of RNA polymerase Nihon Rinsho. Japanese Journal of Clinical Medicine 29(9): 2167-2176
Vorob'eva, N.V.; Nebrat, L.T.; Potapov, V.A.; Romashchenko, A.G.; Salganik, R.I. 1982: Reverse transcription of heterologous RNA by RNA-dependent DNA polymerase from Escherichia coli Molekuliarnaia Biologiia 16(5): 977-983
Kamzolova, S.G.; Ozolin', O.N. 1981: Role of RNA polymerase in ensuring fidelity in copying the template during transcription in E. coli Biokhimiia 46(4): 717-722
Skolnik-David, H.; Aloni, Y. 1983: Pausing of RNA polymerase molecules during in vivo transcription of the SV40 leader region EMBO Journal 2(2): 179-184
Cerovská, N.; Petrzik, K.; Moravec, T.; Mráz, I. 1998: Potato virus A detection by reverse transcription-polymerase chain reaction Acta Virologica 42(2): 83-85
Klausa, V.I.; Nivinskas, R.G. 1988: Determination of the direction of the transcription of bacteriophage T4 genes by heat induction of the transcription from recombinant plasmid Pl-promoter: 2 directions in the region of genes 25-29 Genetika 24(1): 42-52