Isoelectric spectra of liver tissue and blood serum albumin for rabbits of different age
Sopkina, D.A.; Ostolovskiĭ, E.M.; Ivlev, V.N.
Ukrainskii Biokhimicheskii Zhurnal 50(6): 730-734
1978
ISSN/ISBN: 0201-8470 PMID: 34911 Document Number: 128526
The isoelectric spectrum of albumin isolated from the liver and blood serum of 30-, 45- and 90-day old rabbits was studied by isoelectric focusing in the B-borate buffer-mannitol system. The liver albumin displays heterogeneity and is separated into 4-5 fractions, with pI 4.8-6.0. Age peculiarities are found in the isoelectric spectrum of this protein. The serum albumin spectrum for rabbits of the age groups studied is characterized by a homogeneous peak with pI 5.59, 5.57 and 5.47 corresponding to 30, 45 and 90 days of age, respectively. Identity of serum albumin and protein of some liver albumin components is established by analyzing the pattern of the isoelectric spectrum for a mixture of preparations of 90 day old rabbit proteins.