Ribonuclease activity of the "myofibrillar" fraction of normal and autolyzed muscle tissue
Nikitina, Z.K.; Shishkin, S.S.; Khoroshkov, I.A.; Debov, S.S.
Biulleten' Eksperimental'noi Biologii i Meditsiny 85(5): 533-537
1978
ISSN/ISBN: 0365-9615 PMID: 656599 Document Number: 126954
The separation in a sucrose gradient of the "myofibrillar" fraction of normal and autolysed muscle tissue gave 4 components. During the post-mortem destruction of the tissue there was observed a slight decrease of the "myofibrillar" fraction yield and also certain changes in the distribution of protein between different components. Under the selected conditions the RNA-ase activity was found in all 4 components. During the course of autolysis the enzymatic activity increased in the whole "myofibrillar" fraction, as well as in the lysosomal-mitochondrial components of "myofibrillae".