Fluorescence histochemical studies of the uptake of exogenous noradrenaline and dopamine by in vitro cultured cerebrocortex explants of the rat
Pfister, C.; Goworek, K.
Zeitschrift für Mikroskopisch-Anatomische Forschung 91(3): 521-535
1977
ISSN/ISBN: 0044-3107 PMID: 616685 Document Number: 122439
Cerebrocortex of the neonatal rats were cultivated (--14 days). The cultures were studied living and with histological and fluorescence histochemical methods. A differentiation of neuronal cell- and fiber elements, oligodendro glial cells and astrocytes was found. The glyoxylic acid technique to estimate biogenic monoamines (Lindvall et al. 1974) was adapted up the cultivated explants. The normal cultures have only 24 h post cultivationem a specific fluorescence granularly in small concentration of the surface of the explant and in the explant self. Incubations with noradrenaline and dopamine demonstrated a various accumulation of the exogenous transmitters in the various parts of the cultivated explants. Uptake and releasing mechanisms in the cultivated material of the cerebrocortex were discussed with respect to the results of the sympathetic ganglia in vitro.