Effect of shenfu injection on the synthesis of pulmonary surfactant in cultured lung explants
Luo, Z-qiang.; Feng, D-dan.; Yue, S-jie.
Hunan Yi Ke Da Xue Xue Bao 28(2): 137-140
2003
ISSN/ISBN: 1000-5625 PMID: 12934358 Document Number: 565593
Objective To explore the effect of shenfu injection on pulmonary surfactant synthesis. Methods Lung explants of adult rats were cultured in free serum DMEM. (3H) choline incorporated into lung tissue was determined by liquid scintillation system. The contents of total phospholipid (TPL) and phosphatidylcholine (PC) in lung tissue were measured by inorganic phosphorus quantitative analysis and thin-layer chromatography, respectively. Results Shenfu injection enhanced (3H) choline incorporation in a dose-dependent manner and increased the contents of TPL and PC as well as the ratio of PC/TPL. Both protein kinase C (PKC) antagonist H7 and calmodulin antagonist W7 inhibited the effects of Shenfu injection. The ginsenosides, ginsenoside Rb1, and ginsenoside Rg1 promoted the (3H) choline incorporation. Conclusion Shenfu injection can enhance the synthesis of pulmonary surfactant through pathways mediated by PKC and calmodulin. The ginsenoside is an effective component in Shenfu injection.