Serum and duodenal alkaline phosphatase levels in fed and fasted magnesium deficient rats
Neafsey, P.J.; Schwartz, R.
Journal of Nutrition 107(6): 1061-1067
1977
ISSN/ISBN: 0022-3166 PMID: 864516 Document Number: 121944
Rats depleted of Mg for 13 days were killed after 18 to 20 h of starvation or 2 h after they had eaten a standard-sized meal. Serum and duodenal alkaline phosphatase (AP) were less in starved Mg-depleted rats than in pair-fed and freely fed controls. With refeeding, serum alkaline phosphatase values rose significantly in all groups, but the values in refed Mg-depleted rats remained below those of starved control rats. Inhibition of AP activity with L-phenylalanine and urea suggested that the differences in AP values of starved and fed rats were mainly due to enzymes of intestinal origin. Intestinal AP values increased after feeding in all groups, but were proportionately greatest in Mg-depleted rats. The pattern of serum and duodenal AP in fed and starved rats and the results of polyacrylamide disc gel electrophoresis in duodenal AP extracts suggest that Mg depletion alters the release of AP into the blood which normally occurs in response to feeding.