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Topological asymmetry of phospholipids in membranes

Bergelson, L.D.; Barsukov, L.I.

Science 197(4300): 224-230

1977

ISSN/ISBN: 0036-8075
PMID: 327544
Document Number: 121373
Methods available for studying transversal phospholipid distribution in artificial and biological membranes fall into 5 general categories: chemical labeling; NMR spectroscopy with shift and broadening reagents; enzymatic splitting by phospholipase; protein-mediated phospholipid exchange; and immunochemical methods. The application of these techniques to studies of the topological asymmetry of phospholipids in natural and artificial membranes is discussed.

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