Molecular control of B cell triggering by antigen-specific T cell-derived helper factor
Shiozawa, C.; Singh, B.; Rubinstein, S.; Diener, E.
Journal of Immunology 118(6): 2199-2205
1977
ISSN/ISBN: 0022-1767 PMID: 68082 Document Number: 121232
Soluble, carrier-specific helper factor extracted from for use as hapten-carrier conjugates to stimulate in vitro anti-TNP immune responses, it was possible to define the conditions for optimal T cell help as one at which the carrier antigenic determinants are presented to the bone marrow-derived (B) cell in a closely spaced sequence. Such a carrier probably focuses helper factor molecules by providing an array of determinants to which they can bind and then interact in a concentrated fashion with a small area of the B cell surface membrane. When comparing the capacity of B cells to cooperate with helper factor from syngeneic or allogeneic T cells, it was found that for a successful immune response to occur, both cooperating elements have to be of syngeneic origin. This extends the concept of allogeneic restriction between cooperating B and T cells to the level of interaction of soluble T cell-derived helper factor with B cells.