Antigen-specific helper factors present in the supernatant of concanavalin A-induced spleen cell cultures
Kilburn, D.G.; Anaka, R.
Journal of Immunology 127(2): 702-705
1981
ISSN/ISBN: 0022-1767 PMID: 6166684 Document Number: 172639
The supernatants from cultures of concanavalin A-induced spleen cells contained both antigen-specific and nonspecific (Interleukin 2) helper factors (Hf). The antigen-specific factor could be isolated from the supernatant by adsorption onto and elution from antigen-Sepharose immunoadsorbents. Specific Hf was produced in cultures of either immune or nonimmune spleen cells although in the latter case the quantity of Hf was significantly less. The specific Hf did not manifest the thymocyte stimulatory property of 112.