Interactions of biogenic amines with organic phosphate: a proposed model in vitro for study of biological interactions by 31P nuclear magnetic resonance
Katz, R.; Yeh, H.J.; Johnson, D.F.
Molecular Pharmacology 13(4): 615-620
1977
ISSN/ISBN: 0026-895X PMID: 18659 Document Number: 118895
The interaction between 31P-containing substrates, 4-deoxypyridoxine phosphate and monomethyl phosphate, and a series of protonated biogenic amines was quantitated by use of 31P-1H nuclear magnetic double resonance and titration. The difference in the phosphorus chemical shifts with and without amine in deuterated water indicated that optimal biogenic amine-phosphate interaction appears to require the presence of a catechol ring and .beta.-hydroxyl group in the ethylamine side chain. This is in good agreement with pharmacological measurements in vitro of biogenic amine retention by storage vesicles of rat heart adrenergic nerve terminals, indicating that phosphate could be involved in binding of biogenic amines in these vesicles. The model in vitro presented could provide a rapid, efficient method for biological evaluation of various drug-receptor systems involving phosphate-amine interactions.