Effect of some organosilicon compounds (silatranes) on the biosynthesis of collagen in cartilagenous tissue of chick embryo
Simkhovich, B.Z.; Lukevits, E.Ia.; Zelchan, G.I.; Zamaraeva, T.V.; Mazurov, V.I.
Biokhimiia 42(6): 1128-1133
1977
ISSN/ISBN: 0320-9725 PMID: 196689 Document Number: 117677
The criteria for assessing the intensity of collagen biosynthesis was the formation of peptide bond 14C-hydroxyproline from 14C-proline of incubation medium. All compounds studied (methylsilatrane, ethoxysilatrane and chloromethysilatrane) stimulated total protein biosynthesis and collagen biosynthesis in cartilagenous tissue of chick embryos. The most pronounced stimulation of the biosynthesis of collagen was observed at 3 .cntdot. 10-3 M concentration of silatranes within 180 min of incubation (14C-proline was added to the incubation medium 60 min after the beginning of the incubation). The activity of partly purified collagen prolyl-hydroxylase in the presence of silatranes was also studied.