Tissue engineering of cartilagenous tissue. Vital cryopreservation
Viviente Rodríguez, E.; Sudhoff, H.; Capitán, A.; Torregrosa, C.; Sprekelsen, C.; Bujía, J.
Acta Otorrinolaringologica Espanola 49(4): 269-274
1998
ISSN/ISBN: 0001-6519 PMID: 9707734 Document Number: 497099
The characteristics of in vitro synthesized cartilage tissue using tissue engineering techniques before and after cryopreservation are described. We determined cell survival, growth of extracellular matrix (collagen and proteoglycan) using a computer analysis system, and characterized the cell phenotype with a monoclonal antibody specific for collagen type II. Chondrocytes maintained a differentiated phenotype with collagen and proteoglycan synthesis before and after cryopreservation.