High pressure liquid chromatographic analysis of food for folates
Clifford, C.K.; Clifford, A.J.
Journal - Association of Official Analytical Chemists 60(6): 1248-1251
1977
ISSN/ISBN: 0004-5756 PMID: 924929 Document Number: 114460
Folic acid and its tetrahydro-, 5-methyltetrahydro- and dihydro-derivatives were separated in less than 75 min by chromatography on the anion-exchange resin, Pellionex SAX, with a KCl gradient in phosphate buffer, pH 7.5, as eluent. The smallest amount of folic acid which could be estimated reliably was 35 pmol/10 mu l placed on the column. The contribution of individual forms of folacin to the total content of foods varied widely.