Electron microscopic studies of estrogen-induced ciliogenesis and secretion in uterine tube of the gilt
Nayak, R.K.; Zimmerman, D.R.; Albert, E.N.
American Journal of Veterinary Research 37(2): 189-197
1976
ISSN/ISBN: 0002-9645 PMID: 1259215 Document Number: 109588
Daily s/c doses of 200 mu g 17 beta -oestradiol in maize oil were given to 12 gilts, 3-4 months after ovariectomy. Four similar gilts received only maize oil. Tissue samples from the fimbriated end of the uterine tube were processed for light and electron microscopy at intervals of 1-7 days after initiation of treatment. Nine electron micrographs are presented. Epithelial cells were initially atrophied and contained no cilia, and controls remained in this state. After two days of treatment, fibrogranular aggregates appeared in the perinuclear and apical cytoplasm and in association with the nuclear envelope, and procentrioles were formed round the diplosomal centriole, probably through acentriolar basal body replication. After three days of treatment, the endoplasmic reticulum (ER) was prominent and was associated with proliferative elements, and mitochondria, polyribosomes and generative complexes (associations between procentrioles and condensation forms) were numerous. Condensation forms changed to hollow spheres filled with growing, radially-arranged procentrioles, which were assembling their microtubules. Mature basal bodies became aligned along the luminal surface of the cell. Numbers of cilia increased between days 3-7. Probably oestrogen stimulates replication of basal bodies, while fibrogranular aggregates are formed in the nucleus and pass out through the nuclear envelope. Secretory cells showed maximum differentiation of rough ER after three days treatment, and secretory granules increased from days 3-7, indicating increased protein synthesis. Low conception rates reported after hormonal synchronization of oestrus indicate that a delicate hormone balance is required for maximum ciliogenesis and secretion.