Preparation and storage of control slides for fetal hemoglobin determination by acid elution
Barr, J.K.; Shafer, J.A.
American Journal of Medical Technology 42(1): 54-56
1976
ISSN/ISBN: 0002-9335 PMID: 1247026 Document Number: 106084
To simplify the reading of acid eluted blood smears for fetal hemoglobin, positive and negative controls are placed side by side on a single slide. Red blood cells having high Hb F content are stained a bright pink in contrast to cells devoid of Hb F which appear as ghost cells with a faint pink outline. A method for preparing, fixing, and short-term storing of control slides with their subsequent room temperature acid elution and staining is given.