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Preparation and testing of different antibrucellic vaccines

Durand, M.; Lingelser, J.; Loquerie, R.; Prunet, P.

Developments in Biological Standardization 31: 362-376

1976

ISSN/ISBN: 0301-5149
PMID: 1261748
Document Number: 105314
From a highly virulent Brucella melitensis strain of human origin, different vaccines of good antigenic quality are obtained, ranging from the very agglutinogenic oily type vaccine to the totally non-agglutinogenic oily type vaccine. The non-agglutinogenic vaccine is obtained by an original method consisting of submission of the original brucella to several subcultures in the presence of a small quantity of antiserum and clonal selection of a population. The strain finally selected is totally non-agglutinogenic and its biochemical characteristics are hardly different from those of the original strain; it conserves good antigenicity and compares favourably with the other non-agglutinogenic vaccines tested.

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