Aminoacyl-tRNA formation in the liver and skeletal muscles of rabbits under the influence of multiple injections of ACTH alone and in combination with sodium ribonucleinate
Germaniuk, I.L.; Goĭdash, M.M.
Problemy Endokrinologii 22(2): 83-86
1976
ISSN/ISBN: 0375-9660 PMID: 179083 Document Number: 102178
A study was made of the action mechanism of ACTH. ACTH (10 Units/kg) alone or in combination with sodium ribonucleinate (10 mg/kg) was injected to rabbits for 14 days, daily. Sum total tRNA and aminoacyl-tRNA-synthetases were extracted from the liver and the skeletal muscles. tRNA acetylation with lysine-1-C14, leucine-1-C14 and alanine-1-C14 was investigated in vitro in homologous systems. It was found that repeated ACTH injections led to a decrease in the intensity of aminoacyl-tRNA formation in the liver and the skeletal muscles. When ACTH was injected in combination with sodium ribonucleinate formation of aminoacyl-tRNA was much more intensive in both organs than when ACTH was injected alone.