A modified method for isolating alpha-amylase from rabbit skeletal muscle
Krzyzanowski, M.
Archivum Immunologiae et Therapiae Experimentalis 24(5): 777-783
1976
ISSN/ISBN: 0004-069X PMID: 999479 Document Number: 100952
A modified method for purifying alpha-amylase from rabbit skeletal muscle is described, which in comparison with the previously published method gives better results of isolation and is economical, less time-consuming, and require less material. The former method gave 1,340-fold purification of the enzyme with a yield of 0-6%, and the new method 2,014-fold purification and 7-6% yield. Electrophoresis in polyacrylamide gel showed absence of a strictly defined zone of enzyme activity and presence of inactive proteins.