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Integration and transfection of an arbovirus by mammalian cells

Zhdanov, V.M.; Azadova, N.B.

Molekuliarnaia Biologiia 10(6): 1296-1302

1976

ISSN/ISBN: 0026-8984
PMID: 1053085
Document Number: 99893
A system: L cells chronically infected with Sindbis virus was studied. Unlike acute infection wherein the mature virions are produced, the chronically infected tissue culture produces subviral structures-infectious ribonucleoproteins. Molecular hybridization experiments revealed the integration of the viral genome (DNA-transcript) into the cellular genome. Transfection experiments showed the possibility to induce the synthesis of the virus in sensitive cells treated with DNA from the chronically infected cells.

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