Restricted integration into genomic DNA following transfection of Chinese-hamster ovary cells with N-acetylgalactosamine-4-sulphatase cDNA
Webb, E.
Biotechnology and Applied Biochemistry 23(3): 205-207
1996
ISSN/ISBN: 0885-4513 PMID: 8679105 Document Number: 460081
The effect of using different conditions of electroporation for introducing the gene coding for human N-acetylgalactosamine-4-sulphatase into Chinese-hamster ovary (CHO) cells has been studied in an effort to increase the yield of recombinant protein. In each case the range of enzyme activities in the medium of transfected cells proved to be similar, with genetic analysis of a higher producer revealing only one gene copy integrated. Hence, allowing for position effects, the number of gene copies stably integrated into the CHO genome of all transfectants presumably was low, potentially a single copy in each. This inability to allow high-copy-number integration may suggest a constraint on the levels of this protein which can be tolerated by the cell.