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The morphological and functional integrity of cryo-preserved leukocytes

Truog, P.; Lauffenburger, A.L.; Gudat, F.; Nagel, G.; Albrecht, R.; Kaspar, M.

Schweizerische Medizinische Wochenschrift 106(22): 765-768

1976

ISSN/ISBN: 0036-7672
PMID: 1087047
Document Number: 98811
Human peripheral blood lymphocytes were frozen by a simplified method which is described. Light and electron microscopic investigations of the frozen-thawed cells showed no significant morphological changes compared to the fresh cells; comparison of E-rosette formation and nonspecific acid esterase activity before and after cryoconservation afford evidence of a possible slight change in the percent relationship of T- and B-cells, whereas thymidine uptake values within normal range after stimulation with PHA and homologous cells demonstrate the functional integrity of the lymphocytes.

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