Mechanism of biogenic monoamine stimulation of the enzymatic reduction of disulfides
Kulinskiĭ, V.I.; Kolesnichenko, L.S.
Ukrains'kyi Biokhimichnyi Zhurnal 48(6): 683-689
1976
ISSN/ISBN: 0041-610X PMID: 189471 Document Number: 98483
A possibility is shown to study the catecholamine (CA) action mechanism on the simple and available object, the liver supernatant fraction. Under these conditions the physiological concentrations of CA increase the disulphide reductase (DSR) activity. The effect of adrenaline, noradrenaline and isadrine (isopropylnoradrenaline) is realized through beta-receptors, the mesaton (phenylephrine) effect is realized through receptors differing from the classic ones. Serotonin stimulates DSR too but in an order higher concentration; this effect is blocked by beta-adrenolytic. For CA action the membrane factor is necessary, as the effect takes place only at protracted homogenization or preliminary solubilization by detergents. The additional data are obtained in favour of the hypothesis on the DSR activation through the chain: adenylate cyclase-3',5'-AMP-protein kinase.