Effect of crataemon on the respiration of isolated mitochondria
Ivancheva, E.; Tomov, T.; Russanov, E.
Acta Physiologica et Pharmacologica Bulgarica 2(2): 35-41
1976
ISSN/ISBN: 0323-9950 PMID: 827923 Document Number: 98424
Crataemon, a drug containing the total mixture of flavonoids isolated from the leaves of Crataegus minogina, caused activation and inhibition of the respiration of isolated rat liver and heart mitochondria, with different time and dose dependence and with different sensitivity to EDTA. The activating effect which developed immediately, caused by small doses of crataemon, was lacking in uncoupled mitochondria and was completely abolished by EDTA. The inhibitory effect developed immediately or after the activation phase. It was caused by higher doses, with the degree and the latency period dependent on the dose. This effect was due to the action of crataemon on the flavoprotein-cytochrome c section of the electron transport chain. The inhibitory effect was partly abolished by EDTA. These effects were explained by the presence of at least 2 crataemon components: EDTA-sensitive and EDTA-insensitive. The EDTA-sensitive component was determined as a Zn-flavonoid complex. In small doses it uncoupled oxidative phosphorylation, while large doses inhibited the electron transport between flavoprotein and cytochrome c. The EDTA-insensitive component caused only inhibition of the electron transport in the same part of the chain and manifested its effect in the higher doses.