Changes of the phospholipid bilayer structure under the adsorption of ferricytochrome c on its surface
Obraztsov, V.V.; Kobelev, V.S.; Tenchov, B.I.; Selishcheva, A.A.; Sibel'dina, L.A.
Biokhimiia 41(11): 2015-2020
1976
ISSN/ISBN: 0320-9725 PMID: 191102 Document Number: 97907
Changes in the mobility of phospholipid molecules in liposomes membranes under adsorption ferricytochrome c on its surface were studied by means of NMR and EPR spectroscopy. It is found that the interaction of cytochrome molecules with vesicles causes the broadening of 1H-NMR signals of hydrophobic as well as polar groups in cardiolipin and phosphatidylcholine in the presence of lauric or phosphatidic acid. This broadening of 1H-NMR signals in hydrophobic groups may be caused by decrease in the rate of lateral diffusion of phospholipid molecules. The changes in the correlation time of hydrophobic spin-proub in liposomes containing phosphatydiloholine and cardiolipin with the increase of ferricytochrome c concentration were also observed. These changes suggest that the formation of protein-phospholipid clusters results in the impair of the regular structure of phospholipid bilayer.