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Electron microscopic detection of blood group antigen A on human erythrocytes by means of ferritin- and gold-labelled protectin of helix pomatia

Wagner, M.; Wagner, B.

Zeitschrift für Immunitatsforschung Experimentelle und Klinische Immunologie 151(2): 117-125

1976

ISSN/ISBN: 0300-872X
PMID: 134531
Document Number: 96354
By means of ferritin- and Au-labeled protectin from the albumin gland of H. pomatia the blood group antigen A was located on human erythrocytes of groups A1, A2 and A1B. With erythrocytes of groups O and B, the reaction is negative. The antigen is focally distributed on the outer surface of the cell membrane. Cells from groups A1 and A1B have an antigen A concentration about 4 times greater than A2 cells. The numbers of particles after tagging with ferritin or Au are comparable. The Au particles show an extremely high contrast and are therefore very suitable for the immunoelectron microscopic localization of antigens.

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