Electron microscopic study of the surface of nonirradiated and UV-irradiated human blood lymphocytes
Krylenkov, V.A.; Brudnaia, M.S.; Komissarchik, I.I.
Tsitologiia 25(4): 476-479
1983
ISSN/ISBN: 0041-3771 PMID: 6879717 Document Number: 216527
The action of the short-wave ultraviolet radiation (UV, 254 nm) upon the outer perimembrane layers (OPML) of the human blood lymphocytes was studied with the aid of electron microscopy. The alcian blue and ruthenium red dye was used for the contrasting of the OPML. The UV-exposure of cells in shown to lead to the diminution of the dye-adsorbing cell surface as well as to the change of the nature of the observed OPML structures. The problem of the connection between the structural changes of the cell surface with modification of antigen properties of immunocompetent cells is discussed.