Automated separation and measurement of lactate dehydrogenase isoenzymes
Busby, M.G.; Kudirka, P.J.; Carey, R.N.; Toren, E.C.
Clinical Chemistry 22(4): 468-470
1976
ISSN/ISBN: 0009-9147 PMID: 1253429 Document Number: 96257
We describe a totally automated, computer-controlled system for separating and measuring the activity of lactate dehydrogenase (EC 1.1.1.27) isoenzymes. These isoenzymes in tissue extracts were separated on disposable DEAE-cellulose mini-columns. Resolution was complete except that LD-1 was not resolved from LD-2.