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Assay for citrovorum factor (NSC-3590) in the presence of methotrexate (NSC-740)

Mehta, B.M.; Hutchison, D.J.

Cancer ChemoTherapy Reports 59(5): 935-937

1975

ISSN/ISBN: 0069-0112
PMID: 1081908
Document Number: 95467
Treatment of certain malignancies with high-dose methotrexate/citrovorum factor rescue has recently been adopted as an effective regimen. A microbiologic assay capable of detecting citrovorum factor in the presence of massive amounts of methotrexate has been developed using a strain of Pediococcus cerevisiae resistant to methotrexate. The assay described in this paper is an inexpensive and rapid method of studying the distribution kinetics of citrovorum factor.

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