Effect of theophylline on binding of delta-9-tetrahydrocannabinol with mammalian neuronal and non-neuronal membranes
Ghosh, S.K.; Poddar, M.K.
Indian Journal of Experimental Biology 31(2): 130-135
1993
ISSN/ISBN: 0019-5189 PMID: 8388853 Document Number: 940
Delta-9-tetrahydrocannabinol (THC) (1.6 times 10-6-13.33 times 10-6 M) binds to neuronal and non-neuronal subcellular membranes in a biphasic manner. Its binding to neuronal membranes occur in the following order synaptosome gt myelin gt brain microsome and brain mitochondria. Unlike brain membranes binding of delt-9-THC is greater with liver microsome than liver mitochondria. Irrespective of membranes the ophylline (Th) (11.0 times 10-6 and 550.5 times 10-6 M) increases the binding of delta 9-THC by exposure of more number of delta-9-THC binding sites on the subcellular membranes without affecting its binding affinity. Failure of Th (11.0 times 10-6 - 550.0 times 10-6 M) to increase the binding of delta-9-THC (1.6 times 10-6 - 13.33 times 10-6 M) in solubilized membranes suggests the involvement of membrane lipid in the Th-induced enhancement of delta-9-THC binding.
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