The physiology and biochemistry of streptomycetes. 1. Isolation, identification and investigations of microbial synthesis of stereoisomers in the paromomycin complex
Reuter, G.; Liebermann, B.; Köster, H.
Die Pharmazie 30(11): 733-736
1975
ISSN/ISBN: 0031-7144 PMID: 1219776 Document Number: 92900
The antibiotics contained in the paromomycin (I) complex prepared from Streptomyces albus var. metamycinus var. nov. were isolated and identified. For the practical isolation of the stereoisomers with following hydrolytic decomposition, N-acetylation was undertaken. The chief components were N-pentaacetyl I.sbd.I (.dbd. I.sbd.B) and its stereoisomer, N-pentaacetyl I.sbd.II (I .dbd. I.sbd.C); there were also 2 compounds present in the acetylated mixture corresponding to these but with additional acetylation at an OH group. DL-valine in the nutrient medium gave rise to an increase in the biologically less active II portion at the expense of the formation of the I.sbd.I compound. The I.sbd.II sulfate had only 32% of the inhibiting action towards Bacillus subtilis ATCC 6633 as had the I.sbd.I compound. These non-acetylated compounds were obtained by using the method of Machr and Schaffner.