The physiology and biochemistry of streptomycetes. 2. Chemical decomposition and isolation of paromomycin decomposition products
Liebermann, B.; Köster, H.; Reuter, G.
Die Pharmazie 30(12): 796-798
1975
ISSN/ISBN: 0031-7144 PMID: 1219799 Document Number: 91387
N-pentaacetylparomomycin-I previously obtained was split by 3N HCl hydrolysis (refluxing at 95.degree. C for 2.5 h) into 4 fragments: D-ribose (I), D-glucosamine (II), 2-desoxyastreptamine (III), and paromose I (=neosamine B) (IV). These compounds were separated by cation exchange resin Dowex 50- WX8 (H+) and later isolated by column chromatography, in which I was eluted with H2O and II with 1 N HCl. III was separated from IV and parommobiosamine present in the hydrolysate in small amounts. Separation was carried out by an HCl gradient of 0.5 N to 2 N NCl in the order, II, III, IV. Since IV-HCl was not crystallizable, it was obtained as the N-diAc derivative for gravimetric determination. These compounds often obtained in amounts of only a few milligrams were sufficiently pure for radioactive studies.