Feeding, Egg-Laying, and Embryology of the Columbia Lance Nematode, Hoplolaimus columbus
Fassuliotis, G.
Journal of Nematology 7(2): 152-158
1975
ISSN/ISBN: 0022-300X PMID: 19308148 Document Number: 92879
Feeding and egg-laying of Hoplolaimus columbus were observed on excised lucerne (cultivar 'DuPuits) root cultures in 1.0% nutrient agar. Feeding was ectoparasitic on cortical cells in the maturation zone of the root. Oocytes were first observed in the anterior ovary of the feeding female 8 to 9 days after feeding began. Globular secretions emanating from the vagina and vulva preceded migration of the posterior-most oocyte of the anterior gonad into the columella. The egg shell was formed within 8 hours, and the egg was laid within 12 to 24 hours. The eggs differed from those of other plant-parasitic nematodes in having a stalk on the distal end. The average time required from egg-laying to hatching was 12 days in water and lucerne root cultures. [AS].