A quantitative method for determination of aflatoxin B in roasted corn
Shannon, G.M.; Shotwell, O.L.
Journal - Association of Official Analytical Chemists 58(4): 743-745
1975
ISSN/ISBN: 0004-5756 PMID: 1150613 Document Number: 92753
Roasting aflatoxin-contaminated corn will reduce toxin levels. A quantitative analysis for aflatoxin in roasted corn has been developed by modifying a cleanup technique for green coffee extracts approved as official first action by the AOAC. A chloroform extract is partially purified on a Florisil column, and thin layer chromatographic (TLC) plates are developed with methylene chloride-chloroform-isoamyl alcohol-formic acid (81+15+3+1). Recoveries average 101% and the sensitivity limit is 5 ppb aflatoxin B1. A 2-dimensional TLC procedure can also be used to separate the aflatoxins from background interferences.