Quantitative fluorodensitometric measurements of aflatoxin B 1 with a flying-spot densitometer. I. Fluorodensitometric study of the behavior of aflatoxin B 1 standard spots on different types of silica gel
Beljaars, P.R.; Fabry, F.H.
Journal - Association of Official Analytical Chemists 55(4): 775-780
1972
ISSN/ISBN: 0004-5756 PMID: 5056226 Document Number: 50748
TLC plates were scanned after development with a reflectance flying-spot densitometer. The procedure for routine measurements of standard B1 spots is described herein. Three different types of silica gel layers were spotted with standard aliquots of aflatoxin B1 covering a range of 0.4–20 ng. Silica gel H plates were developed with a mixture of chloroform– trichloroethylene–n-amyl alcohol–formic acid (80 + 15 + 4 + 1), while silica gel G and MNG- HR plates were developed with a mixture of chloroform-acetone (90 + 10). Sharp and defined separation patterns for anatoxins B1, B2, G1, and G2 were recorded on H and MN-G-HR plates, while unsatisfactory separation was found on G plates. The relationship between peak area and concentration was linear for II and MN-G-IIR plates, but not for G plates. Variations of ±15–20% were calculated among the fluorescence of standard B1 spots between plates with same sorbent layers. Coefficients of variation of ±5–7% for single densitometric measurements on one plate under normal routine conditions were calculated for a series of 3 or 4 standard aflatoxin B1 spots ranging from 1 to 4 ng on H and MN-GHR plates. Coefficients of variation of ±11–12% were calculated for G plates. Results obtained in this investigation are compared with those found by previous investigators.