Immunoassay for Soluble RANKL (Receptor Activator of NF-ĸB Ligand) in Serum

Hawa, G.; Brinskelle, N.-Schmal; Glatz, K.; Maitzen, S. and Wolfgang Woloszczuk

Clinical Laboratory 49(9-10): 461-463

2003


ISSN/ISBN: 1433-6510
PMID: 14572201
Document Number: 9132
This paper describes a method for the direct measurement of human sRANKL (receptor activator of NF-B ligand), a cytokine of the tumor necrosis factor superfamily, which is a key player in bone metabolism. Its role in the regulation of osteogenic disorders such as osteoporosis, Paget's disease and rheumatoid arthritis is being extensively discussed in the literature at present. We developed a highly specific, simple and reliable ELISA which allows the direct measurement of uncomplexed sRANKL in human serum. Assay characteristics such as analytical precision, sensitivity, interfering factors, sample stability and dilution linearity are shown. Reference values from healthy volunteers (n=57) were found to be between 0 and 2.7 pmol/l (10th – 90th percentile) with a mean serum value of 1.3 pmol/l (median 0.9 pmol/l).

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Immunoassay for Soluble RANKL (Receptor Activator of NF-ĸB Ligand) in Serum