Use of peroxidase markers in the research and diagnosis of paludism
Gentilini, M.; Richard-Lenoble, D.
Bulletin de la Societe de Pathologie Exotique et de Ses Filiales 68(2): 193-197
1975
ISSN/ISBN: 0037-9085 PMID: 1242696 Document Number: 88556
The indirect peroxidase labelled technic for the diagnosis and research in malaria rests on the same principle than immunofluorescence test (I. F.). Homologous or heterologous antigens are incubated with patient serum in the same conditions than for I. F., and then with peroxydase, labelled, conjugate diluted 1/200. The resulting complex is colored by the diaminobenzidine method of Graham and Karnovski. The coloration is observed in light microscopy. This technic as sensitive as immunofluorescence test can be done, with a cheaper microscopic material. The possibility of studying the conjugate fixation in electron microscopy opens new direction for research.